Identical small subunit ribosomal RNA gene nucleotide sequence of bovine Theileria isolates (Korea and Japan) and Theileria buffeli (Marula, Kenya)
J S Chae,1O D Kwon,2P J Holman,1S D Waghela,1G G Wagner,1
and J M Lee2
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A & M University, College Station, TX, USA.
Received September 22, 1997; Accepted December 02, 1997.
Small subunit ribosomal RNA (SSU rRNA) gene nucleotide sequences of bovine Theileria isolates from Korea (KLS and KCB) and Japan (JHS) were determined. The genes from each isolate were amplified by the polymerase chain reaction and the approximately 1.8 kb product cloned and sequenced by a modified dideoxynucleotide method. Overlapping gene segments produced with a series of primers were sequenced, resulting in a complete DNA sequence for both forward and reverse strands of the SSU rRNA genes of each isolate. SSU rRNA gene sequences (termed Type A) were identical among the bovine Theileria isolates from Korea and the isolate from Japan. A GenBank data library homology search showed the sequence to be the same as that listed as Theileria buffeli isolated from cattle in Marula, Kenya.
Fig. 1 Agarose gel stained with 2% ethidium bromide of amplified small subunit ribosomal RNA (18S rRNA) genes from a bovine Theileria laboratory stock from Changsu-gun Chonbuk, Korea (KLS), a bovine Theileria sp. from Kimje-gun Chonbuk, Korea (KCB), and a bovine Theileria sp. from Shintoku in Hokkaido, Japan (JHS). The first lane contains a 1 kb DNA ladder marker (Gibco-BRL).
Fig. 2 Sequence alignment of the Type A small subunit ribosomal RNA gene sequence (GenBank Accession) No. U97047) from the Korean (KLS and KCB) and Japanese (JHS) Theileria isolates with the corresponding gene sequence from Theileria buffeli from GenBank database (GenBank Accession No. Z15106).
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